Human LILRB4 (Leukocyte Immunoglobulin Like Receptor Subfamily B Member 4) CLIA Kit
The Human LILRB4 (Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 4) CLIA Kit is a cutting-edge assay designed for the precise measurement of LILRB4 levels in human biological samples such as serum, plasma, and cell culture supernatants. This innovative kit is known for its superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.LILRB4 is a key protein belonging to the immunoglobulin-like receptor family, playing a crucial role in immune regulation and receptor signaling.
This protein is implicated in various immune-related diseases and disorders, making it a valuable biomarker for investigating inflammatory conditions, autoimmune diseases, and cancer. The Human LILRB4 CLIA Kit offers researchers a reliable tool to study the role of LILRB4 in disease pathogenesis and potentially identify new therapeutic targets.
Product Name:
Human LILRB4 (Leukocyte Immunoglobulin Like Receptor Subfamily B Member 4) CLIA Kit
SKU:
HUES00609
Target:
Human LILRB4 (Leukocyte Immunoglobulin Like Receptor Subfamily B Member 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LILRB4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LILRB4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LILRB4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LILRB4. You can calculate the concentration of Human LILRB4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
98-112
93-106
Average (%)
95
105
99
1:4
Range (%)
92-107
88-100
98-115
Average (%)
98
93
105
1:8
Range (%)
91-105
99-115
101-114
Average (%)
98
107
106
1:16
Range (%)
87-99
93-109
87-98
Average (%)
93
99
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
91
EDTA plasma (n=5)
90-104
95
Cell culture media (n=5)
98-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.12
302.09
989.45
93.83
284.25
990.58
Standard deviation
10.08
29.0
77.97
8.31
32.86
109.46
C V (%)
10.17
9.6
7.88
8.86
11.56
11.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LILRB4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LILRB4 in samples. No significant cross-reactivity or interference between Human LILRB4 and analogues was observed.
