The Human LIPC (Lipase, Hepatic) ELISA Kit is a powerful tool for the precise measurement of lipase levels in human serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Lipase, hepatic is an important enzyme involved in lipid metabolism, specifically in the breakdown of fats in the liver. Dysregulation of lipase activity has been linked to various health conditions such as obesity, fatty liver disease, and pancreatic disorders.
Therefore, this ELISA kit is essential for studying the role of lipase in these diseases and developing potential therapeutic interventions.With the Human LIPC ELISA Kit, researchers can confidently analyze lipase levels in biological samples with precision and efficiency, making it an invaluable tool for advancing our understanding of lipid metabolism and related health conditions.
Product Name:
Human LIPC (Lipase, Hepatic) ELISA Kit
SKU:
HUES01682
Target:
Human LIPC (Lipase, Hepatic)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LIPC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LIPC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LIPC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LIPC. You can calculate the concentration of Human LIPC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
93-109
91-108
Average (%)
100
100
98
1:4
Range (%)
87-103
80-93
83-94
Average (%)
94
86
88
1:8
Range (%)
94-106
82-93
89-100
Average (%)
100
88
95
1:16
Range (%)
90-105
80-93
86-97
Average (%)
96
85
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
97
EDTA plasma (n=5)
84-97
91
Cell culture media (n=5)
94-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.04
3.8
0.52
1.12
3.64
Standard deviation
0.03
0.06
0.2
0.03
0.06
0.19
C V (%)
6.12
5.77
5.26
5.77
5.36
5.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LIPC concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LIPC in samples. No significant cross-reactivity or interference between Human LIPC and analogues was observed.
