Human LL-37 (Antibacterial Protein LL-37) ELISA Kit
The Human LL-37 Antibacterial Protein ELISA Kit is a cutting-edge tool for the precise measurement of LL-37 levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for various research purposes.LL-37, also known as cathelicidin antimicrobial peptide, is a key player in the body's defense against bacterial infections, exhibiting potent antimicrobial properties. Its role in the immune response and inflammatory processes makes it a valuable target for studying infectious diseases and developing novel therapeutic strategies.
With its ability to accurately quantify LL-37 levels, this ELISA kit opens up new possibilities for investigating the impact of LL-37 in various health conditions and exploring avenues for potential treatments. Invest in the Human LL-37 Antibacterial Protein ELISA Kit to advance your research and unlock the secrets of this crucial antimicrobial protein.
Product Name:
Human LL-37 (Antibacterial Protein LL-37) ELISA Kit
SKU:
HUES03304
Target:
Human LL-37 (Antibacterial Protein LL-37)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LL-37. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LL-37 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LL-37, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LL-37. You can calculate the concentration of Human LL-37 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
86-100
87-98
Average (%)
91
92
92
1:4
Range (%)
93-106
85-98
89-103
Average (%)
99
91
94
1:8
Range (%)
88-100
81-94
85-99
Average (%)
94
86
92
1:16
Range (%)
91-103
83-98
87-98
Average (%)
97
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.72
12.01
44.23
4.99
11.72
45.29
Standard deviation
0.25
0.69
2.43
0.31
0.48
1.39
C V (%)
5.3
5.75
5.49
6.21
4.1
3.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LL-37 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LL-37 in samples. No significant cross-reactivity or interference between Human LL-37 and analogues was observed.
