Human Low Density Lipoprotein Receptor Related Protein 8 (LRP8) ELISA Kit
The Human Low Density Lipoprotein Receptor-Related Protein 8 (LRP8) ELISA Kit is a powerful tool for the precise measurement of LRP8 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it suitable for a variety of research applications.LRP8, also known as Apolipoprotein E Receptor 2 (APOER2), is a vital protein involved in lipid metabolism and the regulation of cellular signaling pathways. Dysregulation of LRP8 has been implicated in various diseases, including neurodegenerative disorders, cardiovascular diseases, and cancer.
Therefore, monitoring LRP8 levels can provide valuable insights into the pathogenesis of these conditions and facilitate the development of targeted therapeutic strategies.Overall, the Human LRP8 ELISA Kit offers researchers a reliable and efficient means of studying the role of LRP8 in human physiology and disease, paving the way for advancements in personalized medicine and drug discovery.
Product Name:
Human Low Density Lipoprotein Receptor Related Protein 8 (LRP8) ELISA Kit
Product Code:
HUDL01758
Size:
96 Assays
Target:
LRP8
Synonyms:
APOER2, Apolipoprotein E Receptor 2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
