Human LOX-1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1) CLIA Kit
The Human LOX-1 (Lectin-like oxidized low-density lipoprotein receptor 1) CLIA Kit is specifically designed for the precise measurement of LOX-1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for various research purposes.LOX-1 is a key receptor involved in oxidized low-density lipoprotein (oxLDL) uptake and contributes to the development of atherosclerosis and cardiovascular diseases.
Monitoring LOX-1 levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of therapeutic strategies targeting LOX-1.With its high-performance capabilities, the Human LOX-1 CLIA Kit is an essential tool for researchers studying cardiovascular diseases, atherosclerosis, and related disorders. Its user-friendly format and robust performance make it a valuable asset for investigating LOX-1 biology and its potential as a target for novel therapies.
Product Name:
Human LOX-1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1) CLIA Kit
SKU:
HUES00926
Target:
Human LOX-1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LOX-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LOX-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LOX-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LOX-1. You can calculate the concentration of Human LOX-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
92-108
95-112
Average (%)
105
100
102
1:4
Range (%)
94-109
95-109
98-114
Average (%)
101
103
106
1:8
Range (%)
92-107
101-118
98-111
Average (%)
99
109
105
1:16
Range (%)
92-105
88-102
88-104
Average (%)
98
95
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
93-107
98
Cell culture media (n=5)
96-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.38
236.59
872.66
91.46
230.02
934.35
Standard deviation
10.25
27.35
54.1
7.8
23.6
78.3
C V (%)
10.53
11.56
6.2
8.53
10.26
8.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LOX-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LOX-1 in samples. No significant cross-reactivity or interference between Human LOX-1 and analogues was observed.
