The Human LOX (Lysyl Oxidase) CLIA Kit is specially designed for the precise quantification of LOX levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.LOX is an important enzyme involved in the cross-linking of collagen and elastin fibers, contributing to the structural integrity of the extracellular matrix.
Dysregulation of LOX has been associated with various diseases, such as fibrosis, cancer, and cardiovascular disorders, highlighting its significance as a biomarker for investigating these conditions and exploring potential therapeutic interventions.Don't miss out on the opportunity to advance your research with the Human LOX CLIA Kit from Assay Genie. Order now and unlock new insights into LOX biology and its implications in health and disease.
Product Name:
Human LOX (Lysyl Oxidase) CLIA Kit
SKU:
HUES00149
Target:
Human LOX (Lysyl Oxidase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LOX. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LOX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LOX, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LOX. You can calculate the concentration of Human LOX in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
102-117
98-109
Average (%)
91
109
103
1:4
Range (%)
96-113
86-98
88-102
Average (%)
104
91
93
1:8
Range (%)
97-113
89-102
103-117
Average (%)
103
95
109
1:16
Range (%)
99-116
88-101
98-112
Average (%)
106
94
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
101-116
107
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.56
1.5
3.84
0.54
1.52
3.98
Standard deviation
0.07
0.14
0.43
0.04
0.17
0.26
C V (%)
12.5
9.33
11.2
7.41
11.18
6.53
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LOX concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LOX in samples. No significant cross-reactivity or interference between Human LOX and analogues was observed.
