The Human Lptn (XCL1) Lymphotactin CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of lymphotactin levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit provides reliable and reproducible results, making it a valuable tool for researchers in a variety of fields.Lymphotactin, also known as XCL1, is a chemokine involved in the regulation of immune responses and inflammation. It plays a key role in recruiting immune cells to sites of infection or injury, making it a crucial biomarker for studying immune system function and inflammatory processes.
Understanding the levels of lymphotactin in biological samples can provide valuable insights into various diseases and conditions, making this kit essential for research in immunology, inflammation, and related fields.Overall, the Human Lptn (XCL1) Lymphotactin CLIA Kit offers researchers a powerful tool for accurately measuring lymphotactin levels and advancing our understanding of immune responses and inflammation in human samples.
Product Name:
Human Lptn/XCL1 (Lymphotactin) CLIA Kit
SKU:
HUES00947
Target:
Human Lptn/XCL1 (Lymphotactin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Lptn/XCL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Lptn/XCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Lptn/XCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Lptn/XCL1. You can calculate the concentration of Human Lptn/XCL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-114
95-109
101-118
Average (%)
107
101
108
1:4
Range (%)
102-118
86-99
91-106
Average (%)
109
91
97
1:8
Range (%)
91-107
94-110
100-114
Average (%)
98
100
108
1:16
Range (%)
87-100
86-98
101-116
Average (%)
92
92
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
108
EDTA plasma (n=5)
89-100
95
Cell culture media (n=5)
87-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.22
108.63
402.71
49.2
100.74
428.5
Standard deviation
5.88
11.47
44.54
5.29
9.29
48.55
C V (%)
12.45
10.56
11.06
10.75
9.22
11.33
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Lptn/XCL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Lptn/XCL1 in samples. No significant cross-reactivity or interference between Human Lptn/XCL1 and analogues was observed.
