Human LRG1 (Leucine Rich Alpha-2-Glycoprotein 1) ELISA Kit
The Human LRG1 (Leucine Rich Alpha-2-Glycoprotein 1) ELISA Kit is a specialized assay designed for the quantitative detection of LRG1 levels in human biological samples. LRG1 is a glycoprotein associated with various physiological and pathological conditions, and its quantification can provide insights into inflammatory responses, immune modulation, and disease pathogenesis.
Accurate measurement of LRG1 levels is essential for understanding its role in the body and its potential as a biomarker for different health conditions. Our LRG1 ELISA Kit offers high sensitivity and specificity, ensuring accurate and reproducible results. With stringent quality control measures in place, this kit provides reliable performance and user-friendly protocols, making it suitable for both research and clinical applications. Trust Assay Genie's LRG1 ELISA Kit for precise and dependable quantification of this important biomarker in your studies.
Product Name:
Human LRG1 (Leucine Rich Alpha-2-Glycoprotein 1) ELISA Kit
SKU:
AEES00103
Target:
Human LRG1 (Leucine Rich Alpha-2-Glycoprotein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LRG1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LRG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LRG1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LRG1. You can calculate the concentration of Human LRG1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-105
89-99
95-105
Average (%)
100
92
99
1:4
Range (%)
82-93
97-113
95-110
Average (%)
90
105
103
1:8
Range (%)
87-94
85-99
89-102
Average (%)
91
91
97
1:16
Range (%)
88-96
83-99
87-96
Average (%)
92
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
93
EDTA plasma (n=5)
95-108
102
Cell culture media (n=5)
89-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.85
4.51
18.91
1.85
4.88
17.0
Standard deviation
0.1
0.21
0.94
0.1
0.24
0.75
C V (%)
5.41
4.66
4.97
5.41
4.92
4.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LRG1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LRG1 in samples. No significant cross-reactivity or interference between Human LRG1 and analogues was observed.
