Human LRP6 (Low Density Lipoprotein Receptor Related Protein) CLIA Kit
The Human LRP6 (Low-Density Lipoprotein Receptor-Related Protein) CLIA Kit is specifically designed for the accurate measurement of LRP6 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.LRP6 is a key protein involved in the Wnt signaling pathway, which plays a critical role in cell proliferation, differentiation, and development. Dysregulation of this pathway has been implicated in various diseases including cancer, osteoporosis, and Alzheimer's disease, highlighting the importance of LRP6 as a biomarker for these conditions.
By using the Human LRP6 CLIA Kit, researchers can study the expression and regulation of LRP6 in different disease states, potentially leading to the development of novel therapeutic strategies. This kit is a valuable tool for advancing our understanding of the role of LRP6 in human health and disease.
Product Name:
Human LRP6 (Low Density Lipoprotein Receptor Related Protein) CLIA Kit
SKU:
HUES00693
Target:
Human LRP6 (Low Density Lipoprotein Receptor Related Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LRP6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LRP6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LRP6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LRP6. You can calculate the concentration of Human LRP6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
89-104
91-106
Average (%)
95
95
97
1:4
Range (%)
99-115
85-97
97-114
Average (%)
105
91
105
1:8
Range (%)
90-105
90-100
87-101
Average (%)
98
95
93
1:16
Range (%)
92-105
101-114
102-115
Average (%)
99
108
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
100
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
219.26
413.78
1933.85
207.02
383.82
1917.28
Standard deviation
19.27
47.71
150.84
25.82
31.32
162.78
C V (%)
8.79
11.53
7.8
12.47
8.16
8.49
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LRP6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LRP6 in samples. No significant cross-reactivity or interference between Human LRP6 and analogues was observed.
