The Human LT-Beta (Lymphotoxin Beta) CLIA Kit is a highly sensitive assay designed for the accurate quantification of LT-Beta levels in human samples such as serum, plasma, and cell culture supernatants. With its high specificity and reliability, this kit provides consistent and reproducible results, making it an invaluable tool for various research applications.LT-Beta, also known as lymphotoxin beta, is a key player in the immune system, regulating inflammation and immune responses. Its dysregulation has been implicated in various diseases, including autoimmune disorders, chronic inflammation, and cancer.
Therefore, measuring LT-Beta levels can provide valuable insights into disease pathogenesis and aid in the development of potential therapeutic interventions.Utilizing cutting-edge technology, the Human LT-Beta CLIA Kit offers researchers a precise and efficient method for studying LT-Beta levels in human samples, advancing our understanding of immune responses and inflammatory processes. Order your kit today and unlock new possibilities in your research endeavors.
Product Name:
Human LT beta (Lymphotoxin Beta) CLIA Kit
SKU:
HUES00945
Target:
Human LT beta (Lymphotoxin Beta)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LTβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LTβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LTβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LTβ. You can calculate the concentration of Human LTβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
99-114
96-109
Average (%)
101
107
102
1:4
Range (%)
92-105
93-105
99-117
Average (%)
98
100
107
1:8
Range (%)
92-103
85-97
96-112
Average (%)
97
92
102
1:16
Range (%)
91-104
98-110
90-105
Average (%)
97
104
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
100-113
105
Cell culture media (n=5)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.53
41.86
246.67
28.6
38.63
228.74
Standard deviation
2.92
4.34
25.26
2.77
2.93
15.49
C V (%)
10.61
10.37
10.24
9.69
7.58
6.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LTβ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LTβ in samples. No significant cross-reactivity or interference between Human LTβ and analogues was observed.
