Human LT betaR (Lymphotoxin Beta Receptor) CLIA Kit
The Human LT-BetaR (Lymphotoxin Beta Receptor) CLIA Kit is specifically designed for the accurate measurement of LT-BetaR levels in human serum, plasma, and cell culture supernatants. This highly sensitive and specific kit ensures precise and dependable results, making it an excellent tool for research in various fields.LT-BetaR is a key receptor involved in the immune response and inflammation, playing a critical role in diseases such as autoimmune disorders, inflammatory bowel disease, and chronic inflammatory conditions.
By measuring LT-BetaR levels, researchers can gain valuable insights into these conditions and develop targeted therapies for addressing them effectively.Overall, the Human LT-BetaR CLIA Kit offers a reliable and efficient solution for studying LT-BetaR expression levels and understanding its role in various physiological and pathological processes.
Product Name:
Human LT betaR (Lymphotoxin Beta Receptor) CLIA Kit
SKU:
HUES00313
Target:
Human LT betaR (Lymphotoxin Beta Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LTβR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LTβR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LTβR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LTβR. You can calculate the concentration of Human LTβR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
91-107
90-103
Average (%)
91
99
95
1:4
Range (%)
94-107
101-115
88-104
Average (%)
99
107
95
1:8
Range (%)
99-113
99-111
93-106
Average (%)
105
105
99
1:16
Range (%)
86-97
88-102
89-104
Average (%)
92
95
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
91
EDTA plasma (n=5)
99-116
108
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
195.26
403.94
1376.58
195.96
386.93
1413.13
Standard deviation
21.09
36.19
89.62
17.17
30.53
153.47
C V (%)
10.8
8.96
6.51
8.76
7.89
10.86
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LTβR concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human LTβR in samples. No significant cross-reactivity or interference between Human LTβR and analogues was observed.
