Human Lysosomal Associated Membrane Protein 2 (LAMP2) ELISA Kit
The Human LAMP2 (Lysosomal-Associated Membrane Protein 2) ELISA Kit is a powerful tool for measuring LAMP2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results that are suitable for a wide range of research applications.LAMP2 is a vital protein found in lysosomes, playing a crucial role in autophagy and the degradation of cellular components. Dysregulation of LAMP2 has been linked to various diseases, including lysosomal storage disorders and neurodegenerative conditions.
As a key biomarker, studying LAMP2 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human LAMP2 ELISA Kit is a valuable tool for researchers seeking to understand the role of LAMP2 in health and disease, providing reliable and accurate measurements for impactful research outcomes.
Product Name:
Human Lysosomal Associated Membrane Protein 2 (LAMP2) ELISA Kit
Product Code:
HUDL01680
Size:
96 Assays
Target:
LAMP2
Synonyms:
CD107-B, CD107b, LAMP-2C, LAMPB, LGP96, Lysosome-associated membrane glycoprotein 2, CD107 antigen-like family member B
Detection Method:
Sandwich
Range:
62.5-4,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
