Human Macrophage Inflammatory Protein 1 Alpha (MIP1a) ELISA Kit
The Human Macrophage Inflammatory Protein-1 Alpha (MIP-1A) ELISA Kit is a powerful tool for accurately measuring the levels of MIP-1A in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit delivers reliable and reproducible results, making it suitable for a variety of research applications.MIP-1A is a key player in the inflammatory response, attracting immune cells to sites of infection or injury. It is involved in various diseases and conditions, including inflammatory disorders, autoimmune diseases, and infectious diseases.
Therefore, detecting MIP-1A levels can provide valuable insights into disease processes and potential therapeutic interventions.With the Human MIP-1A ELISA Kit, researchers can uncover important information about immune responses and inflammatory pathways, advancing our understanding of various health conditions and paving the way for the development of novel treatments.
Product Name:
Human Macrophage Inflammatory Protein 1 Alpha (MIP1a) ELISA Kit
Product Code:
HUDL01856
Size:
96 Assays
Target:
MIP1a
Synonyms:
CCL3, MIP1-A, SCYA3, Chemokine C-C-Motif Ligand 3, Small Inducible Cytokine A3, Homologous To Mouse Mip-1a, Tonsillar Lymphocyte LD78 Alpha Protein
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
