Human Major Histocompatibility Complex Class I B (MHCB) ELISA Kit
The Human Major Histocompatibility Complex Class I B (MHCB) ELISA Kit is a powerful tool for researchers looking to accurately quantify levels of MHCB in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results that can be used for a variety of research applications.MHCB plays a crucial role in the immune system by presenting antigens to T cells, thereby triggering immune responses against foreign pathogens and cancer cells.
Dysregulation of MHCB expression has been linked to various autoimmune diseases, infections, and cancer, underscoring the importance of studying MHCB levels in human samples.With the Human MHCB ELISA Kit, researchers can easily measure MHCB levels in their samples, providing valuable insights into immune responses and diseases. This kit is user-friendly, versatile, and backed by AssayGenie's commitment to quality and excellence in scientific research.
Product Name:
Human Major Histocompatibility Complex Class I B (MHCB) ELISA Kit
Product Code:
HUDL01846
Size:
96 Assays
Target:
MHCB
Synonyms:
MHC-B, HLA-B, HLAB, AS, SPDA1, Leukocyte Antigen B, Ankylosing Spondylitis, HLA class I histocompatibility antigen, B-7 alpha chain
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
