Human Major Histocompatibility Complex Class II DQ Beta 1 (MHCDQb1) ELISA Kit
The Human Major Histocompatibility Complex Class II DQ Beta 1 (MHC DQB1) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of MHC DQB1 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for research in immunology, autoimmunity, and transplantation. The MHC DQB1 molecule plays a crucial role in antigen presentation and immune response regulation.
Dysregulation of MHC DQB1 has been linked to various autoimmune diseases, including rheumatoid arthritis, multiple sclerosis, and type 1 diabetes. This ELISA kit enables researchers to study the expression levels of MHC DQB1 and its impact on immune system function, offering valuable insights into disease mechanisms and potential therapeutic targets.
Product Name:
Human Major Histocompatibility Complex Class II DQ Beta 1 (MHCDQb1) ELISA Kit
Product Code:
HUDL01848
Size:
96 Assays
Target:
MHCDQb1
Synonyms:
HLA-DQB1, HLADQb1, CELIAC1, HLA-DQB, IDDM1, HLA Class II Histocompatibility Antigen,DQ Beta 1 Chain
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-100
90
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
