Human MANF (Mesencephalic Astrocyte Derived Neurotrophic Factor) CLIA Kit
The Human MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor) CLIA Kit is a cutting-edge assay designed for the accurate measurement of MANF levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring precise and consistent results for a wide range of research applications.MANF is a critical neurotrophic factor with diverse functions in the central nervous system, including neuroprotection, neurogenesis, and neuronal survival.
Dysregulation of MANF has been implicated in various neurological disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its advanced technology and reliable performance, the Human MANF CLIA Kit is an invaluable tool for researchers and clinicians seeking to unravel the complexities of neurodegenerative diseases and explore new avenues for treatment. Discover the power of precision with this innovative assay kit from Assaygenie.
Product Name:
Human MANF (Mesencephalic Astrocyte Derived Neurotrophic Factor) CLIA Kit
SKU:
HUES00359
Target:
Human MANF (Mesencephalic Astrocyte Derived Neurotrophic Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MANF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MANF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MANF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MANF. You can calculate the concentration of Human MANF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
86-98
88-103
Average (%)
91
91
94
1:4
Range (%)
88-100
84-97
90-103
Average (%)
95
91
95
1:8
Range (%)
100-113
100-119
88-102
Average (%)
106
108
94
1:16
Range (%)
96-113
101-116
96-113
Average (%)
104
109
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-113
106
EDTA plasma (n=5)
100-116
107
Cell culture media (n=5)
85-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.36
94.31
338.64
37.27
99.93
341.63
Standard deviation
3.44
11.16
36.17
3.51
9.45
26.92
C V (%)
8.97
11.83
10.68
9.42
9.46
7.88
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MANF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MANF in samples. No significant cross-reactivity or interference between Human MANF and analogues was observed.
