The Human Mannose Receptor C-Type 1 (MRC1) ELISA Kit is a powerful tool for the quantitative detection of MRC1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.MRC1 is a key protein involved in the immune response, acting as a pattern recognition receptor that plays a crucial role in pathogen recognition and clearance. It is also involved in the regulation of inflammatory responses and antigen presentation.
Abnormal levels of MRC1 have been associated with various diseases including autoimmune disorders, infectious diseases, and cancer, making it a valuable biomarker for understanding these conditions and developing potential therapeutic strategies.With its high-performance features and broad applicability, the Human Mannose Receptor C-Type 1 (MRC1) ELISA Kit is an essential tool for researchers investigating the role of MRC1 in health and disease.
Product Name:
Human Mannose Receptor C Type 1 (MRC1) ELISA Kit
Product Code:
HUDL01902
Size:
96 Assays
Target:
MRC1
Synonyms:
CD206, CLEC13D, CLEC13DL, MRC1L1, C-type lectin domain family 13 member D-like, Macrophage mannose receptor 1-like protein 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
