Human Mannosidase Alpha Class 1A Member 1 (MAN1A1) ELISA Kit
The Human MAN1A1 (Mannosidase Alpha Class 1A Member 1) ELISA Kit is specifically designed for the precise detection of MAN1A1 levels in human serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent choice for various research applications.MAN1A1 is an essential enzyme involved in glycoprotein processing, playing a critical role in cellular functions and protein quality control. Dysregulation of MAN1A1 has been implicated in various diseases, including lysosomal storage disorders and cancer, highlighting its significance as a potential diagnostic and therapeutic target.
This ELISA kit enables researchers to measure MAN1A1 levels with precision, providing valuable insights into its biological functions and potential implications in disease pathways. Whether investigating glycoprotein metabolism or exploring novel therapeutic interventions, the Human MAN1A1 ELISA Kit offers a reliable tool for advancing scientific research.
Product Name:
Human Mannosidase Alpha Class 1A Member 1 (MAN1A1) ELISA Kit
Product Code:
HUDL01781
Size:
96 Assays
Target:
MAN1A1
Synonyms:
HUMM3, HUMM9, MAN9, Mannosyl-Oligosaccharide 1,2-Alpha-Mannosidase IA, Mannosidase alpha class 1A member 1, Processing alpha-1,2-mannosidase IA
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
