The Human MAOB (Monoamine Oxidase B) ELISA Kit is specifically designed for the accurate detection of MAOB levels in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides precise and reliable results for a variety of research applications.MAOB is an important enzyme involved in the degradation of neurotransmitters such as dopamine and serotonin. Dysregulation of MAOB activity has been linked to various neurological disorders, including Parkinson's disease and depression.
By measuring MAOB levels, researchers can gain valuable insights into these conditions and potentially develop new therapeutic approaches.Overall, the Human MAOB ELISA Kit is an essential tool for studying the role of MAOB in health and disease, offering a comprehensive solution for researchers in the field of neurobiology and beyond.
Product Name:
Human MAOB (Amine oxidaseB) ELISA Kit
SKU:
HUES03225
Target:
Human MAOB (Amine oxidaseB)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MAOB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MAOB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAOB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MAOB. You can calculate the concentration of Human MAOB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
95-110
94-111
Average (%)
92
103
102
1:4
Range (%)
89-102
80-95
88-103
Average (%)
96
87
95
1:8
Range (%)
91-105
82-95
87-98
Average (%)
98
87
92
1:16
Range (%)
86-102
85-98
81-94
Average (%)
93
91
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
98
EDTA plasma (n=5)
85-99
91
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
227.34
432.72
1676.03
240.1
460.19
1548.46
Standard deviation
15.19
25.83
79.61
12.92
22.69
57.14
C V (%)
6.68
5.97
4.75
5.38
4.93
3.69
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MAOB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MAOB in samples. No significant cross-reactivity or interference between Human MAOB and analogues was observed.
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