Human Matrix Metalloproteinase 19 (MMP19) ELISA Kit
The Human Matrix Metalloproteinase 19 (MMP19) ELISA Kit is a cutting-edge tool for the precise measurement of MMP19 levels in human biological samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers dependable and reproducible results, making it an invaluable asset for a diverse array of research endeavors.MMP19 is a key enzyme involved in extracellular matrix remodeling, playing a vital role in tissue repair, inflammation, and various pathological processes.
Its dysregulation is linked to numerous diseases, including cancer, arthritis, and cardiovascular disorders, underscoring its significance as a biomarker for understanding disease mechanisms and developing therapeutic interventions.Whether investigating the molecular mechanisms of disease or evaluating potential therapeutic targets, the Human MMP19 ELISA Kit offers researchers a powerful tool for insightful and accurate analysis, paving the way for groundbreaking discoveries and advancements in biomedical research.
Product Name:
Human Matrix Metalloproteinase 19 (MMP19) ELISA Kit
Product Code:
HUDL01877
Size:
96 Assays
Target:
MMP19
Synonyms:
RASI-1, MMP18, Occasionally Referred To As Stromelysin-4
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
