Human MBP/MBL (Mannose Binding Protein/Mannose Binding Lectin) ELISA Kit
The Human MBP (Mannose Binding Protein) ELISA Kit from Assay Genie is a reliable tool for detecting levels of MBP in human samples. MBP, also known as Mannose Binding Lectin, is an important protein involved in the innate immune response, binding to sugar molecules on pathogens to facilitate their clearance by the immune system.This ELISA kit offers high sensitivity and specificity, providing accurate and reproducible results for researchers studying immune responses, infectious diseases, and autoimmune disorders.
By measuring MBP levels in serum, plasma, or cell culture supernatants, researchers can gain valuable insights into the role of this protein in various health conditions.Whether investigating immune system function, monitoring disease progression, or developing new therapeutic strategies, the Human MBP ELISA Kit is an invaluable tool for advancing scientific understanding and improving patient outcomes.
Product Name:
Human MBP/MBL (Mannose Binding Protein/Mannose Binding Lectin) ELISA Kit
SKU:
HUES02360
Target:
Human MBP/MBL (Mannose Binding Protein/Mannose Binding Lectin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MBP-C/MBL2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MBP-C/MBL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MBP-C/MBL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MBP-C/MBL2. You can calculate the concentration of Human MBP-C/MBL2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
95-110
98-112
Average (%)
92
103
105
1:4
Range (%)
87-99
84-97
84-97
Average (%)
92
90
91
1:8
Range (%)
89-102
79-90
87-97
Average (%)
96
86
92
1:16
Range (%)
87-102
85-99
85-99
Average (%)
94
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.58
4.58
0.52
1.59
5.01
Standard deviation
0.03
0.07
0.23
0.03
0.07
0.17
C V (%)
5.77
4.43
5.02
5.77
4.4
3.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MBP-C/MBL2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MBP-C/MBL2 in samples. No significant cross-reactivity or interference between Human MBP-C/MBL2 and analogues was observed.
