Human MCAM (Melanoma Cell Adhesion Molecule) CLIA Kit
The Human MCAM (Melanoma Cell Adhesion Molecule) CLIA Kit is a cutting-edge assay designed for the precise measurement of MCAM levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit guarantees accurate and consistent results, making it the perfect choice for various research applications.MCAM is a key molecule involved in melanoma progression, playing a critical role in cell adhesion, migration, and invasion. Its dysregulation has been implicated in the development and metastasis of melanoma, highlighting its significance as a potential therapeutic target and diagnostic marker.
By utilizing the Human MCAM CLIA Kit, researchers can gain valuable insights into the role of MCAM in melanoma and other related diseases, paving the way for innovative treatments and diagnostic strategies. Trust in this advanced assay to accelerate your research and drive meaningful discoveries in the field of oncology.
Product Name:
Human MCAM (Melanoma Cell Adhesion Molecule) CLIA Kit
SKU:
HUES01250
Target:
Human MCAM (Melanoma Cell Adhesion Molecule)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MCAM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCAM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCAM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MCAM. You can calculate the concentration of Human MCAM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
93-107
99-117
Average (%)
104
99
107
1:4
Range (%)
100-116
84-97
98-111
Average (%)
107
91
104
1:8
Range (%)
97-111
91-104
86-100
Average (%)
105
96
91
1:16
Range (%)
96-110
86-98
88-103
Average (%)
103
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
91
EDTA plasma (n=5)
90-103
98
Cell culture media (n=5)
85-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
400.69
1116.07
3628.63
393.97
1188.34
3737.48
Standard deviation
33.26
89.4
361.05
45.23
95.07
340.11
C V (%)
8.3
8.01
9.95
11.48
8.0
9.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MCAM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MCAM in samples. No significant cross-reactivity or interference between Human MCAM and analogues was observed.
