Human MCSP (Melanoma Associated Chondroitin Sulfate Proteoglycan) CLIA Kit
The Human MCSP (Melanoma-Associated Chondroitin Sulfate Proteoglycan) CLIA Kit is specifically designed for the accurate detection of MCSP levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.MCSP is a protein that is associated with melanoma, a type of skin cancer. It is involved in cell adhesion and migration, making it an important biomarker for studying melanoma progression and potentially developing targeted therapies.
By measuring MCSP levels in samples, researchers can gain valuable insights into the mechanisms underlying this disease and potentially identify new therapeutic targets.Overall, the Human MCSP CLIA Kit provides researchers with a powerful tool for investigating the role of MCSP in melanoma and other related conditions, offering a valuable resource for advancing scientific knowledge and potential clinical applications.
Product Name:
Human MCSP (Melanoma Associated Chondroitin Sulfate Proteoglycan) CLIA Kit
SKU:
HUES01257
Target:
Human MCSP (Melanoma Associated Chondroitin Sulfate Proteoglycan)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MCSP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCSP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCSP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MCSP. You can calculate the concentration of Human MCSP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
94-109
94-109
Average (%)
91
101
102
1:4
Range (%)
84-97
102-117
93-106
Average (%)
90
108
98
1:8
Range (%)
101-118
91-108
100-112
Average (%)
108
98
106
1:16
Range (%)
92-106
89-99
85-99
Average (%)
99
94
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
85-99
92
Cell culture media (n=5)
88-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.68
289.81
857.89
97.07
299.56
789.21
Standard deviation
10.04
34.31
71.55
8.51
27.83
64.79
C V (%)
9.5
11.84
8.34
8.77
9.29
8.21
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MCSP concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human MCSP in samples. No significant cross-reactivity or interference between Human MCSP and analogues was observed.
