The Human Melanotransferrin (MFI2) ELISA Kit is a reliable and accurate tool for detecting melanotransferrin levels in human samples. This kit is specifically designed for use with serum, plasma, and cell culture supernatants, providing researchers with precise and reproducible results for a variety of applications.Melanotransferrin, also known as MFI2, is a key protein involved in various physiological processes, including iron metabolism and cell growth regulation. Dysregulation of melanotransferrin has been linked to conditions such as cancer, Alzheimer's disease, and inflammatory disorders, highlighting its importance as a potential biomarker for disease diagnosis and monitoring.
With its high sensitivity and specificity, the Human Melanotransferrin (MFI2) ELISA Kit is an essential tool for researchers looking to study the role of melanotransferrin in health and disease, as well as for the development of novel therapeutic interventions.
Product Name:
Human Melanotransferrin (MFI2) ELISA Kit
Product Code:
HUDL01837
Size:
96 Assays
Target:
MFI2
Synonyms:
CD228, MAP97, MTF1, Antigen P97(Melanoma Associated), Membrane-Bound Transferrin-Like Protein
Detection Method:
Sandwich
Range:
31.25-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
