Human Mercaptopyruvate Sulfurtransferase (MPST) ELISA Kit
The Human Mercaptopyruvate Sulfurtransferase (MPST) ELISA Kit is a reliable and precise tool for the detection of MPST levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results that are essential for various research applications.MPST is a key enzyme involved in sulfur metabolism and redox regulation, playing a crucial role in cellular processes and disease pathways. Dysregulation of MPST has been linked to conditions such as cancer, neurodegenerative diseases, and cardiovascular disorders, making it an important biomarker for understanding these diseases and developing potential therapeutic interventions.
Overall, the Human MPST ELISA Kit is a valuable resource for researchers studying sulfur metabolism, redox signaling, and the role of MPST in human health and disease. Its high performance and versatility make it a must-have tool for investigating the complex mechanisms underlying these conditions.
Product Name:
Human Mercaptopyruvate Sulfurtransferase (MPST) ELISA Kit
Product Code:
HUDL01900
Size:
96 Assays
Target:
MPST
Synonyms:
MST, TST2, Human Liver Rhodanese
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
