The Human MGL (Monoacylglycerol Lipase) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of MGL levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit ensures reliable and reproducible results, making it ideal for a wide range of research applications.MGL is a key enzyme involved in the metabolism of endocannabinoids, playing a crucial role in regulating lipid signaling pathways. Dysregulation of MGL activity has been implicated in various diseases such as inflammation, neurodegenerative disorders, and metabolic diseases, making it a valuable biomarker for studying these conditions and identifying potential therapeutic targets.
By measuring MGL levels in biological samples, researchers can gain valuable insights into the role of this enzyme in disease pathogenesis and potentially identify novel therapeutic strategies. The Human MGL CLIA Kit offers a convenient and efficient solution for studying MGL activity and its implications in human health and disease.
Product Name:
Human MGL (Monoacylglycerol Lipase) CLIA Kit
SKU:
HUES00373
Target:
Human MGL (Monoacylglycerol Lipase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MGL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MGL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MGL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MGL. You can calculate the concentration of Human MGL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
88-99
101-113
Average (%)
92
94
107
1:4
Range (%)
97-113
88-103
85-95
Average (%)
103
94
90
1:8
Range (%)
98-114
85-100
85-99
Average (%)
106
91
90
1:16
Range (%)
87-99
98-110
94-105
Average (%)
94
104
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
98
EDTA plasma (n=5)
93-106
100
Cell culture media (n=5)
93-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.03
290.16
822.18
86.07
280.94
743.11
Standard deviation
7.66
23.94
71.04
8.47
32.28
74.39
C V (%)
8.06
8.25
8.64
9.84
11.49
10.01
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MGL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MGL in samples. No significant cross-reactivity or interference between Human MGL and analogues was observed.
