The Human MHC-E (Major Histocompatibility Complex Class I E) CLIA Kit is specifically designed for the precise detection of MHC-E levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable tool for various research applications.MHC-E is a critical component of the immune system, playing a crucial role in the presentation of antigens to T cells and regulating immune responses.
Dysregulation of MHC-E has been implicated in autoimmune diseases, infectious diseases, and transplant rejection, highlighting its importance as a potential biomarker for studying and developing targeted therapies for these conditions.Overall, the Human MHC-E CLIA Kit offers researchers a reliable and efficient method for measuring MHC-E levels, providing valuable insights into immune responses and potential disease mechanisms.
Product Name:
Human HLA-E CLIA Kit
SKU:
HUES01208
Target:
Human MHCE/HLA-E (Major Histocompatibility Complex Class I E)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MHCE/HLA-E. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MHCE/HLA-E and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MHCE/HLA-E, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MHCE/HLA-E. You can calculate the concentration of Human MHCE/HLA-E in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
92-107
86-97
Average (%)
99
99
92
1:4
Range (%)
103-119
91-102
97-111
Average (%)
109
96
102
1:8
Range (%)
100-116
84-98
101-114
Average (%)
106
91
108
1:16
Range (%)
87-100
87-101
95-108
Average (%)
92
92
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
102
EDTA plasma (n=5)
102-114
107
Cell culture media (n=5)
85-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
204.82
349.57
1715.65
196.52
361.28
1865.04
Standard deviation
24.64
35.13
178.94
23.05
27.06
146.22
C V (%)
12.03
10.05
10.43
11.73
7.49
7.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MHCE/HLA-E concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MHCE/HLA-E in samples. No significant cross-reactivity or interference between Human MHCE/HLA-E and analogues was observed.
