Human MIgamma/CXCL9 (Monocyte Interferon Gamma Inducing Factor) ELISA Kit
The Human MIγ/CXCL9 (Monocyte Interferon Gamma-Inducing Factor) ELISA Kit is a specialized assay intended for the precise quantification of Monocyte Interferon Gamma-Inducing Factor, also known as MIγ or CXCL9, in various biological samples. MIγ/CXCL9 is a chemokine that plays a crucial role in immune responses and cell signaling by attracting monocytes and activated T cells to sites of inflammation. Accurate measurement of MIγ/CXCL9 levels is essential for understanding its role in immune modulation, inflammation, and immune cell recruitment.
This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured with strict adherence to quality control standards, the MIγ/CXCL9 ELISA Kit delivers robust performance while being user-friendly, making it an excellent choice for both research and clinical applications. Researchers can rely on this kit to quantify MIγ/CXCL9 efficiently and effectively in their studies.
Product Name:
Human MIgamma/CXCL9 (Monocyte Interferon Gamma Inducing Factor) ELISA Kit
SKU:
AEES00098
Target:
Human MIγ/CXCL9 (Monocyte Interferon Gamma Inducing Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MIγ/CXCL9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIγ/CXCL9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIγ/CXCL9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MIγ/CXCL9. You can calculate the concentration of Human MIγ/CXCL9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-99
98-108
98-104
Average (%)
94
104
101
1:4
Range (%)
94-104
99-110
100-108
Average (%)
97
102
105
1:8
Range (%)
91-97
87-96
92-102
Average (%)
94
91
97
1:16
Range (%)
98-110
90-102
90-102
Average (%)
102
97
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-101
98
EDTA plasma (n=5)
95-106
99
Cell culture media (n=5)
97-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.83
100.15
451.89
44.84
118.13
456.05
Standard deviation
2.65
4.74
23.18
2.53
5.49
21.21
C V (%)
5.32
4.73
5.13
5.64
4.65
4.65
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MIγ/CXCL9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MIγ/CXCL9 in samples. No significant cross-reactivity or interference between Human MIγ/CXCL9 and analogues was observed.
