Human MIA1 (Melanoma Inhibitory Activity Protein 1) CLIA Kit
The Human MIA1 (Melanoma Inhibitory Activity Protein 1) CLIA Kit is specifically designed for the precise measurement of MIA1 levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers reliable and consistent results, making it an ideal tool for various research purposes.MIA1 is a vital protein known for its role in inhibiting the growth and spread of melanoma, a type of skin cancer.
By detecting and quantifying MIA1 levels, researchers can gain valuable insights into the progression of melanoma and explore potential treatment options. This CLIA kit is essential for studying melanoma and other related diseases, offering valuable information for advancing research and therapeutic strategies.
Product Name:
Human MIA1 (Melanoma Inhibitory Activity Protein 1) CLIA Kit
SKU:
HUES01251
Target:
Human MIA1 (Melanoma Inhibitory Activity Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MIA1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIA1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MIA1. You can calculate the concentration of Human MIA1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
89-105
85-100
Average (%)
94
97
91
1:4
Range (%)
88-100
99-114
98-114
Average (%)
94
105
106
1:8
Range (%)
86-102
92-103
95-109
Average (%)
93
97
103
1:16
Range (%)
84-97
92-104
96-110
Average (%)
91
97
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-103
98
EDTA plasma (n=5)
98-111
104
Cell culture media (n=5)
87-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.07
79.47
189.92
24.31
72.73
187.48
Standard deviation
2.58
6.04
17.38
2.21
5.67
18.84
C V (%)
10.72
7.6
9.15
9.09
7.8
10.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MIA1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MIA1 in samples. No significant cross-reactivity or interference between Human MIA1 and analogues was observed.
