Human Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) ELISA Kit
The Human Microtubule-Associated Protein RP/EB Family Member 1 (MAPRE1) ELISA Kit is specifically designed for the precise detection of MAPRE1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results that are essential for various research applications.MAPRE1 is a critical protein that plays a key role in microtubule dynamics and cell division. Dysregulation of MAPRE1 has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular conditions.
Therefore, the detection of MAPRE1 levels can provide valuable insights for studying these conditions and developing potential therapeutic interventions.Overall, the Human MAPRE1 ELISA Kit from Assay Genie is a valuable tool for researchers seeking to explore the role of MAPRE1 in health and disease, offering reliable and reproducible results to advance scientific understanding in this field.
Product Name:
Human Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) ELISA Kit
Product Code:
HUDL01794
Size:
96 Assays
Target:
MAPRE1
Synonyms:
EB1, Adenomatous Polyposis Coli-Binding protein, APC-binding protein EB1, End-binding protein 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
