Human MIgamma/CXCL9 (Monocyte Interferon Gamma Inducing Factor) CLIA Kit
The Human MIG (CXCL9) CLIA Kit is a specialized assay developed for the precise measurement of monocyte interferon-gamma inducing factor levels in human samples, including serum and plasma. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.MIG, also known as CXCL9, plays a key role in the immune response by recruiting T cells and monocytes to sites of inflammation.
Dysregulation of MIG has been implicated in various inflammatory diseases, including autoimmune disorders and chronic infections. As such, the Human MIG CLIA Kit serves as a valuable tool for investigating these conditions and evaluating potential therapeutic strategies.
Product Name:
Human MIgamma/CXCL9 (Monocyte Interferon Gamma Inducing Factor) CLIA Kit
SKU:
HUES00596
Target:
Human MIgamma/CXCL9 (Monocyte Interferon Gamma Inducing Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MIγ/CXCL9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIγ/CXCL9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIγ/CXCL9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MIγ/CXCL9. You can calculate the concentration of Human MIγ/CXCL9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
90-104
96-111
Average (%)
103
97
104
1:4
Range (%)
96-108
96-107
91-104
Average (%)
101
101
96
1:8
Range (%)
86-101
97-111
89-100
Average (%)
93
104
94
1:16
Range (%)
89-100
93-108
85-95
Average (%)
95
100
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
101
EDTA plasma (n=5)
90-101
95
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.14
286.56
714.86
109.77
302.5
712.59
Standard deviation
8.59
32.27
80.85
9.2
26.68
76.46
C V (%)
8.09
11.26
11.31
8.38
8.82
10.73
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MIγ/CXCL9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MIγ/CXCL9 in samples. No significant cross-reactivity or interference between Human MIγ/CXCL9 and analogues was observed.
