The Human MK (Midkine) CLIA Kit is a highly reliable and sensitive assay designed for the accurate measurement of midkine levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides precise and reproducible results, making it an ideal tool for various research applications.Midkine is a multifunctional growth factor that plays a crucial role in various physiological and pathological processes, including cell growth, differentiation, and migration. Elevated levels of midkine have been linked to cancer, inflammation, and neurodegenerative diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
The Human MK CLIA Kit offers researchers a precise and efficient way to quantify midkine levels, providing valuable insights into the role of this protein in disease progression and potential therapeutic targets. Get reliable and accurate results with this advanced kit from AssayGenie.
Product Name:
Human MK (Midkine) CLIA Kit
SKU:
HUES01193
Target:
Human MK (Midkine)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MK. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MK, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MK. You can calculate the concentration of Human MK in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
86-98
91-107
Average (%)
91
92
98
1:4
Range (%)
96-110
103-116
96-108
Average (%)
103
109
101
1:8
Range (%)
102-119
88-101
89-104
Average (%)
109
95
95
1:16
Range (%)
90-105
95-112
88-99
Average (%)
97
103
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
102
EDTA plasma (n=5)
90-100
95
Cell culture media (n=5)
93-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.9
304.98
911.7
113.64
283.1
904.35
Standard deviation
10.9
32.48
73.94
12.7
29.92
73.79
C V (%)
10.01
10.65
8.11
11.18
10.57
8.16
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MK concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MK in samples. No significant cross-reactivity or interference between Human MK and analogues was observed.
