The Human MK (Midkine) ELISA Kit is specifically designed for the precise measurement of midkine levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit provides reliable and reproducible results, making it an invaluable tool for various research applications.Midkine is a key protein that plays a critical role in cell growth, differentiation, and survival. It is involved in various physiological and pathological processes, including cancer, inflammation, and neurodegenerative diseases.
By accurately measuring midkine levels, researchers can better understand its role in disease development and progression, leading to potential therapeutic advancements. Overall, the Human MK (Midkine) ELISA Kit offers researchers a comprehensive and reliable solution for studying midkine biology and its implications in various diseases, ultimately advancing the field of biomedical research.
Product Name:
Human MK (Midkine) ELISA Kit
SKU:
HUES03174
Target:
Human MK (Midkine)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MK. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MK. You can calculate the concentration of Human MK in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
84-95
90-104
Average (%)
92
90
95
1:4
Range (%)
90-104
83-95
85-97
Average (%)
95
88
91
1:8
Range (%)
87-102
85-98
86-100
Average (%)
93
92
92
1:16
Range (%)
91-103
81-95
83-95
Average (%)
98
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
91
EDTA plasma (n=5)
89-99
94
Cell culture media (n=5)
83-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.49
3.37
0.49
1.34
3.53
Standard deviation
0.04
0.08
0.13
0.03
0.07
0.18
C V (%)
7.69
5.37
3.86
6.12
5.22
5.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MK concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MK in samples. No significant cross-reactivity or interference between Human MK and analogues was observed.
