Human MMP-10 (Matrix Metalloproteinase 10) CLIA Kit
The Human MMP-10 (Matrix Metalloproteinase-10) CLIA Kit is a valuable tool for the accurate measurement of MMP-10 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.MMP-10 is an important enzyme involved in extracellular matrix degradation, playing a key role in tissue remodeling and various pathological processes such as inflammation, cancer metastasis, and tissue repair.
Detection of MMP-10 levels can provide insights into disease progression and potential therapeutic targets.Overall, the Human MMP-10 CLIA Kit is essential for researchers studying the role of MMP-10 in various diseases and for the development of novel treatment strategies targeting this enzyme.
Product Name:
Human MMP-10 (Matrix Metalloproteinase 10) CLIA Kit
SKU:
HUES00119
Target:
Human MMP-10 (Matrix Metalloproteinase 10)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MMP-10. You can calculate the concentration of Human MMP-10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-119
92-106
97-112
Average (%)
108
99
104
1:4
Range (%)
90-106
92-105
88-99
Average (%)
97
100
94
1:8
Range (%)
102-119
101-117
86-96
Average (%)
109
109
91
1:16
Range (%)
99-112
90-105
87-101
Average (%)
105
96
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-104
99
EDTA plasma (n=5)
91-107
97
Cell culture media (n=5)
88-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.66
266.38
764.49
106.35
249.38
782.07
Standard deviation
12.8
27.2
50.92
8.74
27.71
63.66
C V (%)
11.78
10.21
6.66
8.22
11.11
8.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MMP-10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-10 in samples. No significant cross-reactivity or interference between Human MMP-10 and analogues was observed.
