Human MMP-11 (Matrix Metalloproteinase 11) CLIA Kit
The Human MMP-11 (Matrix Metalloproteinase 11) CLIA Kit is specifically designed for the accurate detection of MMP-11 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.MMP-11 is a key enzyme involved in extracellular matrix degradation and remodeling, playing a crucial role in processes such as tissue repair, inflammation, and metastasis.
Abnormal MMP-11 levels have been linked to various diseases including cancer, arthritis, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.Overall, the Human MMP-11 CLIA Kit is a valuable tool for researchers looking to investigate the role of MMP-11 in disease pathogenesis and development, providing accurate and precise measurements for insightful research findings.
Product Name:
Human MMP-11 (Matrix Metalloproteinase 11) CLIA Kit
SKU:
HUES00820
Target:
Human MMP-11 (Matrix Metalloproteinase 11)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MMP-11. You can calculate the concentration of Human MMP-11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-108
100-117
87-100
Average (%)
103
109
92
1:4
Range (%)
91-107
89-101
95-111
Average (%)
97
96
101
1:8
Range (%)
86-99
94-104
89-100
Average (%)
91
99
95
1:16
Range (%)
94-105
98-116
90-104
Average (%)
100
106
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
104
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
100-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.56
282.34
841.22
116.47
301.58
826.08
Standard deviation
12.25
32.84
82.27
9.67
35.19
65.34
C V (%)
11.08
11.63
9.78
8.3
11.67
7.91
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MMP-11 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-11 in samples. No significant cross-reactivity or interference between Human MMP-11 and analogues was observed.
