Human MMP-12 (Matrix Metalloproteinase 12) CLIA Kit
The Human MMP-12 (Matrix Metalloproteinase-12) CLIA Kit is a cutting-edge assay designed for the precise measurement of MMP-12 levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers consistent and dependable results, making it an invaluable tool for a wide array of research endeavors.MMP-12, also known as macrophage elastase, is a key enzyme involved in tissue remodeling and inflammation. Elevated levels of MMP-12 have been linked to various diseases, including chronic obstructive pulmonary disease (COPD), emphysema, and atherosclerosis.
As such, the accurate quantification of MMP-12 is essential for understanding the pathogenesis of these conditions and identifying potential therapeutic targets.By utilizing the Human MMP-12 CLIA Kit, researchers can gain valuable insights into the role of MMP-12 in disease progression and prognosis, ultimately paving the way for the development of innovative treatment strategies. With its robust performance and user-friendly format, this kit is the ideal solution for investigating the intricate mechanisms of MMP-12-mediated pathology.
Product Name:
Human MMP-12 (Matrix Metalloproteinase 12) CLIA Kit
SKU:
HUES00120
Target:
Human MMP-12 (Matrix Metalloproteinase 12)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-12. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-12, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MMP-12. You can calculate the concentration of Human MMP-12 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
85-97
93-106
Average (%)
105
92
100
1:4
Range (%)
93-108
96-109
92-103
Average (%)
100
102
97
1:8
Range (%)
100-113
97-113
98-115
Average (%)
106
104
106
1:16
Range (%)
97-112
92-104
102-117
Average (%)
103
99
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
96-113
103
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
263.96
689.48
2461.65
274.56
670.92
2684.98
Standard deviation
28.53
80.88
149.91
33.47
63.2
202.72
C V (%)
10.81
11.73
6.09
12.19
9.42
7.55
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MMP-12 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-12 in samples. No significant cross-reactivity or interference between Human MMP-12 and analogues was observed.
