Human MMP-17 (Matrix Metalloproteinase 17) CLIA Kit
The Human MMP-17 (Matrix Metalloproteinase 17) CLIA Kit is specifically designed for the precise measurement of MMP-17 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results, making it suitable for various research purposes.MMP-17 is a crucial enzyme that plays a vital role in extracellular matrix remodeling and cell migration. It is implicated in various physiological and pathological processes, including tissue development, wound healing, and cancer metastasis.
Therefore, measuring MMP-17 levels can provide valuable insights into disease progression and potential therapeutic interventions.With this advanced CLIA kit, researchers can effectively study the role of MMP-17 in various biological processes and diseases, paving the way for innovative diagnostic and therapeutic strategies. Get your Human MMP-17 CLIA Kit today and unlock the potential of your research.
Product Name:
Human MMP-17 (Matrix Metalloproteinase 17) CLIA Kit
SKU:
HUES00824
Target:
Human MMP-17 (Matrix Metalloproteinase 17)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-17. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-17, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MMP-17. You can calculate the concentration of Human MMP-17 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
86-100
88-104
Average (%)
90
92
95
1:4
Range (%)
83-99
87-101
102-120
Average (%)
90
93
110
1:8
Range (%)
85-97
91-101
93-107
Average (%)
91
96
98
1:16
Range (%)
86-99
97-110
97-114
Average (%)
91
103
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
92-105
98
Cell culture media (n=5)
96-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.1
299.9
867.3
91.95
282.03
946.19
Standard deviation
12.45
22.85
91.76
8.36
26.26
64.15
C V (%)
12.82
7.62
10.58
9.09
9.31
6.78
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MMP-17 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-17 in samples. No significant cross-reactivity or interference between Human MMP-17 and analogues was observed.
