The Human MMP-2 (Matrix Metalloproteinase 2) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of MMP-2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it valuable for a wide range of research applications. MMP-2 is a key enzyme involved in extracellular matrix degradation, cell migration, and tissue remodeling. Aberrant MMP-2 activity has been linked to various pathological conditions including cancer, cardiovascular diseases, and inflammatory disorders.
Therefore, the Human MMP-2 CLIA Kit serves as a crucial tool for studying these conditions and developing potential therapeutic interventions. With its superior performance and user-friendly design, the Human MMP-2 CLIA Kit from Assay Genie is a valuable asset for researchers seeking to investigate MMP-2 expression and activity in human samples. Visit the provided URL to learn more and order your kit today.
Product Name:
Human MMP-2 (Matrix Metalloproteinase 2) CLIA Kit
SKU:
HUES00822
Target:
Human MMP-2 (Matrix Metalloproteinase 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MMP-2. You can calculate the concentration of Human MMP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
84-97
90-104
Average (%)
94
90
98
1:4
Range (%)
103-120
102-114
91-107
Average (%)
110
108
98
1:8
Range (%)
100-112
89-104
98-115
Average (%)
106
96
106
1:16
Range (%)
91-102
99-111
85-100
Average (%)
96
104
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
104
EDTA plasma (n=5)
88-101
93
Cell culture media (n=5)
98-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.55
3.62
0.55
1.69
3.74
Standard deviation
0.05
0.13
0.29
0.06
0.19
0.24
C V (%)
9.62
8.39
8.01
10.91
11.24
6.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MMP-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-2 in samples. No significant cross-reactivity or interference between Human MMP-2 and analogues was observed.
