Human MMP-24 (Matrix Metalloproteinase 24) CLIA Kit
The Human MMP-24 (Matrix Metalloproteinase 24) CLIA Kit is specifically designed for the accurate quantification of MMP-24 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.Matrix metalloproteinase 24, also known as MMP-24, is a key enzyme involved in the breakdown of the extracellular matrix and regulation of cell behavior. Dysregulation of MMP-24 has been linked to various diseases including cancer, arthritis, and cardiovascular disorders, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.
With the Human MMP-24 CLIA Kit, researchers can confidently study the role of MMP-24 in disease progression and monitor its levels in biological samples with high accuracy and efficiency. This kit is a valuable tool for advancing research in the field of matrix metalloproteinases and their impact on human health.
Product Name:
Human MMP-24 (Matrix Metalloproteinase 24) CLIA Kit
SKU:
HUES00825
Target:
Human MMP-24 (Matrix Metalloproteinase 24)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-24. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-24 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-24, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MMP-24. You can calculate the concentration of Human MMP-24 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
93-107
85-100
Average (%)
92
98
92
1:4
Range (%)
86-99
102-118
95-112
Average (%)
93
109
102
1:8
Range (%)
95-107
99-116
91-106
Average (%)
100
107
97
1:16
Range (%)
90-104
92-107
98-112
Average (%)
95
99
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
106
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
89-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.49
205.28
774.76
112.84
191.68
757.06
Standard deviation
11.3
20.75
59.19
14.09
16.18
65.64
C V (%)
10.51
10.11
7.64
12.49
8.44
8.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MMP-24 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-24 in samples. No significant cross-reactivity or interference between Human MMP-24 and analogues was observed.
