Human MMP-28 (Matrix Metalloproteinase 28) CLIA Kit
The Human MMP-28 (Matrix Metalloproteinase-28) CLIA Kit is a highly reliable and accurate assay for the quantification of MMP-28 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides consistent and reproducible results, making it suitable for a variety of research purposes.Matrix metalloproteinase-28, also known as epilysin, is an enzyme that plays a crucial role in tissue remodeling and wound healing processes.
Dysregulation of MMP-28 has been implicated in various diseases, including cancer, arthritis, and cardiovascular disorders. Therefore, measuring MMP-28 levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of targeted treatments.Overall, the Human MMP-28 CLIA Kit is an indispensable tool for researchers studying the role of MMP-28 in health and disease, offering accurate and precise measurements to support their investigations.
Product Name:
Human MMP-28 (Matrix Metalloproteinase 28) CLIA Kit
SKU:
HUES00871
Target:
Human MMP-28 (Matrix Metalloproteinase 28)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-28. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-28 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-28, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MMP-28. You can calculate the concentration of Human MMP-28 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
100-114
93-108
Average (%)
97
106
98
1:4
Range (%)
94-111
103-120
94-107
Average (%)
102
110
100
1:8
Range (%)
103-115
97-113
87-102
Average (%)
109
104
94
1:16
Range (%)
96-109
86-97
97-114
Average (%)
103
91
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
103
EDTA plasma (n=5)
88-103
94
Cell culture media (n=5)
101-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.74
237.77
920.19
94.69
258.35
870.79
Standard deviation
12.87
26.3
83.19
10.6
28.21
70.1
C V (%)
12.78
11.06
9.04
11.19
10.92
8.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MMP-28 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-28 in samples. No significant cross-reactivity or interference between Human MMP-28 and analogues was observed.
