The Human MMRN1 (Multimerin-1) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of multimerin-1 levels in human samples such as serum, plasma, and cell culture supernatants. This easy-to-use kit provides reliable and reproducible results, making it an ideal tool for researchers in various fields.Multimerin-1 is a glycoprotein involved in platelet function and blood clotting, as well as regulating angiogenesis and inflammation. Dysregulation of multimerin-1 has been implicated in various diseases, including cardiovascular disorders, cancer, and inflammatory conditions.
By measuring multimerin-1 levels, researchers can gain important insights into disease mechanisms and potential therapeutic targets.Overall, the Human MMRN1 CLIA Kit offers researchers a valuable tool for studying the role of multimerin-1 in health and disease, with the potential to advance our understanding of various pathological processes and aid in the development of novel treatment strategies.
Product Name:
Human MMRN1 (Multimerin 1) CLIA Kit
SKU:
HUES00389
Target:
Human MMRN1 (Multimerin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMRN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMRN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMRN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MMRN1. You can calculate the concentration of Human MMRN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
97-113
95-113
Average (%)
101
105
103
1:4
Range (%)
98-114
99-113
97-112
Average (%)
106
107
103
1:8
Range (%)
102-117
91-107
97-109
Average (%)
108
99
103
1:16
Range (%)
96-108
86-101
98-111
Average (%)
101
93
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-115
105
EDTA plasma (n=5)
101-115
107
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.41
126.23
434.08
47.72
116.03
455.7
Standard deviation
5.12
14.52
43.89
6.14
9.27
34.41
C V (%)
10.8
11.5
10.11
12.87
7.99
7.55
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MMRN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMRN1 in samples. No significant cross-reactivity or interference between Human MMRN1 and analogues was observed.
