Human MOG (Myelin Oligodendrocyte Glycoprotein) CLIA Kit
The Human MOG (Myelin Oligodendrocyte Glycoprotein) CLIA Kit is specifically designed for the accurate quantitative measurement of MOG levels in human serum, plasma, and cell culture supernatants. This high-performance kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research purposes.MOG is a key protein found in the myelin sheath of nerve fibers and is essential for maintaining the integrity of the central nervous system. Abnormalities in MOG levels have been linked to autoimmune diseases such as multiple sclerosis, making it a valuable biomarker for understanding these conditions and developing potential treatment strategies.
With its advanced technology and reliable performance, the Human MOG CLIA Kit is an indispensable tool for researchers and clinicians studying neurodegenerative disorders and autoimmune diseases. Trust in this kit to deliver accurate and reproducible results, enabling further advancements in the field of neurology and immunology.
Product Name:
Human MOG (Myelin Oligodendrocyte Glycoprotein) CLIA Kit
SKU:
HUES00398
Target:
Human MOG (Myelin Oligodendrocyte Glycoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MOG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MOG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MOG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MOG. You can calculate the concentration of Human MOG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
96-110
88-102
Average (%)
104
103
94
1:4
Range (%)
100-112
100-112
84-96
Average (%)
105
106
91
1:8
Range (%)
97-115
90-103
97-111
Average (%)
105
95
103
1:16
Range (%)
88-100
88-100
100-116
Average (%)
95
95
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
97
EDTA plasma (n=5)
101-117
107
Cell culture media (n=5)
89-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.28
321.29
863.05
106.61
301.04
799.73
Standard deviation
9.63
27.63
80.78
9.23
25.41
91.73
C V (%)
8.81
8.6
9.36
8.66
8.44
11.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MOG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MOG in samples. No significant cross-reactivity or interference between Human MOG and analogues was observed.
