Human MPIF2 (Myeloid Progenitor Inhibitory Factor 2) CLIA Kit
The Human MPIF2 (Myeloid Progenitor Inhibitory Factor 2) CLIA Kit is a powerful tool for the precise measurement of MPIF2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, delivering accurate and reproducible results that are invaluable for a wide range of research applications.MPIF2 is a key factor involved in regulating myeloid progenitor cells, playing a critical role in immune responses and inflammatory processes. Dysregulation of MPIF2 has been linked to various diseases, including autoimmune disorders and infectious diseases, making it a crucial biomarker for understanding these conditions and developing potential therapeutic strategies.
With its advanced technology and reliable performance, the Human MPIF2 CLIA Kit is an essential tool for researchers exploring the complex mechanisms of immune regulation and inflammatory pathways. Trust in this kit to provide the accurate data you need to advance your research and make significant contributions to the field of immunology.
Product Name:
Human MPIF2 (Myeloid Progenitor Inhibitory Factor 2) CLIA Kit
SKU:
HUES00030
Target:
Human MPIF2 (Myeloid Progenitor Inhibitory Factor 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MPIF2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MPIF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MPIF2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MPIF2. You can calculate the concentration of Human MPIF2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
95-108
101-118
Average (%)
102
102
110
1:4
Range (%)
93-105
94-104
92-106
Average (%)
99
99
100
1:8
Range (%)
93-107
88-102
101-119
Average (%)
100
95
108
1:16
Range (%)
87-98
97-109
91-107
Average (%)
92
102
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
103
EDTA plasma (n=5)
99-113
107
Cell culture media (n=5)
101-117
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.6
304.77
996.69
93.06
320.81
1088.98
Standard deviation
11.62
27.52
95.68
9.46
26.34
72.96
C V (%)
12.28
9.03
9.6
10.17
8.21
6.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MPIF2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MPIF2 in samples. No significant cross-reactivity or interference between Human MPIF2 and analogues was observed.
