Human MRC1 (Mannose Receptor C Type 1 Like Protein 1) CLIA Kit
The Human MRC1 (Mannose Receptor C Type 1-Like Protein 1) CLIA Kit is a highly accurate and reliable tool for the detection of MRC1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.MRC1, also known as Mannose Receptor C Type 1-Like Protein 1, is a crucial protein involved in the immune system, specifically in the recognition and clearance of pathogens and foreign particles.
It plays a key role in immune responses and inflammatory processes, making it a valuable biomarker for studying immune-related disorders and developing potential treatments.With its advanced technology and user-friendly design, the Human MRC1 CLIA Kit is a valuable tool for researchers and clinicians seeking to investigate the role of MRC1 in various health conditions and diseases. Get accurate and reliable results with this high-quality assay kit from AssayGenie.
Product Name:
Human MRC1 (Mannose Receptor C Type 1 Like Protein 1) CLIA Kit
SKU:
HUES01240
Target:
Human MRC1 (Mannose Receptor C Type 1 Like Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MRC1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MRC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MRC1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MRC1. You can calculate the concentration of Human MRC1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
87-102
88-101
Average (%)
90
93
95
1:4
Range (%)
97-115
88-101
96-108
Average (%)
105
95
102
1:8
Range (%)
102-120
84-100
89-103
Average (%)
110
91
95
1:16
Range (%)
99-117
96-112
89-100
Average (%)
108
102
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
102
EDTA plasma (n=5)
100-116
107
Cell culture media (n=5)
98-113
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
221.79
376.39
1986.57
212.03
360.69
1813.6
Standard deviation
25.28
40.76
168.46
21.67
26.01
119.7
C V (%)
11.4
10.83
8.48
10.22
7.21
6.6
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MRC1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MRC1 in samples. No significant cross-reactivity or interference between Human MRC1 and analogues was observed.
