The Human MRC2 (Mannose Receptor C Type 2) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of MRC2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an excellent tool for research applications related to MRC2 expression.MRC2, also known as the Mannose Receptor C Type 2, is a crucial protein involved in various biological processes, including cell adhesion, endocytosis, and extracellular matrix remodeling.
Dysregulation of MRC2 expression has been linked to various diseases, including cancer and fibrosis, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human MRC2 CLIA Kit offers a comprehensive solution for researchers looking to investigate the role of MRC2 in health and disease, providing accurate and reliable data for further scientific exploration.
Product Name:
Human MRC2 (Mannose Receptor C Type 2) CLIA Kit
SKU:
HUES01238
Target:
Human MRC2 (Mannose Receptor C Type 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MRC2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MRC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MRC2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MRC2. You can calculate the concentration of Human MRC2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
86-102
94-108
Average (%)
101
93
102
1:4
Range (%)
94-107
102-115
95-109
Average (%)
102
108
101
1:8
Range (%)
85-96
92-104
91-103
Average (%)
91
99
97
1:16
Range (%)
88-99
89-102
84-99
Average (%)
94
96
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
99
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
90-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
207.98
480.52
1431.39
212.36
457.99
1359.01
Standard deviation
24.56
44.83
113.37
22.28
40.72
130.33
C V (%)
11.81
9.33
7.92
10.49
8.89
9.59
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MRC2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MRC2 in samples. No significant cross-reactivity or interference between Human MRC2 and analogues was observed.
