The Human Melatonin Sulfate ELISA Kit is specifically designed for the precise quantification of melatonin sulfate levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Melatonin sulfate is a metabolite of melatonin, a hormone that regulates the sleep-wake cycle and circadian rhythms in the body.
Its measurement can provide valuable insights into sleep disorders, jet lag, and other conditions related to disrupted melatonin production. By accurately detecting melatonin sulfate levels, researchers can gain a better understanding of these physiological processes and potentially develop targeted interventions for managing sleep-related issues.
Product Name:
Human MS (Melatonin Sulfate) ELISA Kit
SKU:
HUES02714
Target:
Human MS (Melatonin Sulfate)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
2.81 ng/mL
Detection range:
4.69-300 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MS. During the reaction, MS in the sample or standard competes with a fixed amount of MS on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MS. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MS in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
93-110
87-99
Average (%)
100
101
94
1:4
Range (%)
88-102
90-104
94-109
Average (%)
93
97
100
1:8
Range (%)
92-104
89-103
97-113
Average (%)
97
96
104
1:16
Range (%)
87-100
90-100
100-113
Average (%)
92
95
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
102
EDTA plasma (n=5)
91-104
99
Cell culture media (n=5)
89-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.5
20.6
82.2
10.0
22.4
74.2
Standard deviation
0.5
0.9
3.5
0.7
1.3
3.1
C V (%)
5.26
4.37
4.26
7.0
5.8
4.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of MS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes MS in samples. No significant cross-reactivity or interference between MS and analogues was observed.
