Human MSR1 (Macrophage Scavenger Receptor 1) CLIA Kit
The Human MSR1 (Macrophage Scavenger Receptor 1) CLIA Kit is a cutting-edge assay designed for the accurate detection of MSR1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Macrophage Scavenger Receptor 1 (MSR1) is a key protein involved in the immune response and the clearance of pathogens and cellular debris. Dysregulation of MSR1 has been implicated in various diseases including atherosclerosis, Alzheimer's disease, and cancer, highlighting its importance as a potential biomarker for studying these conditions and developing targeted therapies.
With its advanced technology and user-friendly format, the Human MSR1 CLIA Kit from AssayGenie provides researchers with a powerful tool for investigating the role of MSR1 in health and disease, ultimately advancing our understanding of complex biological processes and facilitating the development of novel treatments.
Product Name:
Human MSR1 (Macrophage Scavenger Receptor 1) CLIA Kit
SKU:
HUES00412
Target:
Human MSR1 (Macrophage Scavenger Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MSR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MSR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MSR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MSR1. You can calculate the concentration of Human MSR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
102-120
95-110
Average (%)
102
109
101
1:4
Range (%)
101-114
93-104
99-117
Average (%)
107
98
107
1:8
Range (%)
98-117
96-109
89-103
Average (%)
106
102
95
1:16
Range (%)
90-106
91-104
86-96
Average (%)
96
99
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
92
EDTA plasma (n=5)
87-100
92
Cell culture media (n=5)
94-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
205.7
398.07
1893.94
188.32
425.69
1716.38
Standard deviation
20.26
35.83
199.24
21.24
44.14
159.28
C V (%)
9.85
9.0
10.52
11.28
10.37
9.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MSR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MSR1 in samples. No significant cross-reactivity or interference between Human MSR1 and analogues was observed.
