The Human MSTN (Myostatin) CLIA Kit is a powerful tool for the precise measurement of myostatin levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it suitable for various research purposes.Myostatin is a key regulator of muscle growth and development, inhibiting muscle cell proliferation and differentiation.
Dysregulation of myostatin has been linked to conditions such as muscle wasting disorders, obesity, and metabolic diseases, highlighting its importance as a potential therapeutic target.By utilizing the Human MSTN CLIA Kit, researchers can gain valuable insights into the role of myostatin in various physiological and pathological processes, paving the way for the development of novel treatment strategies for muscle-related disorders.
Product Name:
Human MSTN (Myostatin) CLIA Kit
SKU:
HUES00815
Target:
Human MSTN (Myostatin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MSTN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MSTN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MSTN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MSTN. You can calculate the concentration of Human MSTN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
88-101
94-109
Average (%)
93
95
101
1:4
Range (%)
92-105
100-117
90-105
Average (%)
98
107
97
1:8
Range (%)
89-103
84-100
93-105
Average (%)
96
91
99
1:16
Range (%)
99-113
94-110
90-105
Average (%)
104
102
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
100
EDTA plasma (n=5)
98-111
103
Cell culture media (n=5)
89-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.42
3.52
0.49
1.39
3.69
Standard deviation
0.06
0.17
0.29
0.05
0.16
0.38
C V (%)
12.24
11.97
8.24
10.2
11.51
10.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MSTN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MSTN in samples. No significant cross-reactivity or interference between Human MSTN and analogues was observed.
