The Human MSTN (Myostatin) ELISA Kit is specifically designed for the precise measurement of myostatin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results, which makes it perfect for various research purposes.Myostatin, also known as growth differentiation factor 8 (GDF-8), is a key regulator of muscle growth and development. It inhibits muscle cell proliferation and differentiation, making it a crucial player in muscle atrophy and wasting conditions.
By studying myostatin levels, researchers can gain valuable insights into muscle-related disorders, performance enhancement, and potential therapeutic approaches.With its user-friendly protocol and reliable performance, the Human MSTN ELISA Kit is an indispensable tool for scientists and researchers working in the fields of muscle biology, sports medicine, and neuromuscular diseases. Explore the role of myostatin in health and disease with this cutting-edge assay.
Product Name:
Human MSTN (Myostatin) ELISA Kit
SKU:
HUES02473
Target:
Human MSTN (Myostatin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MSTN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MSTN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MSTN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MSTN. You can calculate the concentration of Human MSTN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
89-105
95-111
Average (%)
97
96
103
1:4
Range (%)
91-106
81-96
86-99
Average (%)
97
88
91
1:8
Range (%)
87-101
82-96
83-95
Average (%)
93
88
88
1:16
Range (%)
94-108
85-98
89-101
Average (%)
99
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
100
EDTA plasma (n=5)
93-107
100
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.34
5.96
19.62
2.23
5.8
18.74
Standard deviation
0.13
0.33
0.98
0.13
0.32
0.89
C V (%)
5.56
5.54
4.99
5.83
5.52
4.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MSTN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MSTN in samples. No significant cross-reactivity or interference between Human MSTN and analogues was observed.
