The Human MT1M (Metallothionein 1M) CLIA Kit is a highly sensitive assay designed for the accurate detection of MT1M levels in human samples such as serum, plasma, and cell culture supernatants. This kit ensures reliable and reproducible results, making it ideal for various research applications.MT1M is a metal-binding protein that plays a crucial role in protecting cells from heavy metal toxicity and oxidative stress. It is involved in various biological processes, including metal homeostasis, detoxification, and cell proliferation.
Additionally, MT1M has been implicated in diseases such as cancer, neurodegenerative disorders, and liver diseases, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Human MT1M CLIA Kit provides researchers with a powerful tool for accurately measuring MT1M levels and investigating its role in various physiological and pathological processes.
Product Name:
Human MT1M (Metallothionein 1M) CLIA Kit
SKU:
HUES00404
Target:
Human MT1M (Metallothionein 1M)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MT1M. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MT1M and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MT1M, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MT1M. You can calculate the concentration of Human MT1M in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
97-113
94-112
Average (%)
102
103
102
1:4
Range (%)
96-112
96-111
90-105
Average (%)
104
104
97
1:8
Range (%)
96-106
95-109
86-100
Average (%)
101
103
92
1:16
Range (%)
88-102
92-107
93-108
Average (%)
95
97
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-110
105
EDTA plasma (n=5)
94-107
102
Cell culture media (n=5)
98-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.55
7.23
20.15
2.57
7.6
18.28
Standard deviation
0.24
0.85
1.67
0.23
0.65
2.06
C V (%)
9.41
11.76
8.29
8.95
8.55
11.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MT1M concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MT1M in samples. No significant cross-reactivity or interference between Human MT1M and analogues was observed.
